lentiviral vector plasmid plcko (Addgene inc)
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Lentiviral Vector Plasmid Plcko, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 34 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/lentiviral+vector+plasmid+plcko/pLCKO+(Plasmid+%2373311)/bio_rxiv__2025__10__02__680088-60-16-20
Average 93 stars, based on 34 article reviews
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Expressing:Article Title: Promoter hijacking by primate LINC00473 disrupts an ancestral CREB-PDE10A feedback loop Article Snippet: qPCR was performed using a CFX96 (Bio-Rad) real time PCR system, with thermal cycling conditions consisting of a 3 minute 95°C initial denaturation, followed by 40 cycles of 95°C C for 10 seconds, 60°C for 20 seconds, and 72°C for 20 seconds, followed by a melt curve step. Clone Assay:Article Title: Promoter hijacking by primate LINC00473 disrupts an ancestral CREB-PDE10A feedback loop Article Snippet: qPCR was performed using a CFX96 (Bio-Rad) real time PCR system, with thermal cycling conditions consisting of a 3 minute 95°C initial denaturation, followed by 40 cycles of 95°C C for 10 seconds, 60°C for 20 seconds, and 72°C for 20 seconds, followed by a melt curve step. Plasmid Preparation:Article Title: Promoter hijacking by primate LINC00473 disrupts an ancestral CREB-PDE10A feedback loop Article Snippet: qPCR was performed using a CFX96 (Bio-Rad) real time PCR system, with thermal cycling conditions consisting of a 3 minute 95°C initial denaturation, followed by 40 cycles of 95°C C for 10 seconds, 60°C for 20 seconds, and 72°C for 20 seconds, followed by a melt curve step. Sequencing:Article Title: Promoter hijacking by primate LINC00473 disrupts an ancestral CREB-PDE10A feedback loop Article Snippet: qPCR was performed using a CFX96 (Bio-Rad) real time PCR system, with thermal cycling conditions consisting of a 3 minute 95°C initial denaturation, followed by 40 cycles of 95°C C for 10 seconds, 60°C for 20 seconds, and 72°C for 20 seconds, followed by a melt curve step. |
